1627Diagnostic Accuracy of Loop-Mediated Isothermal DNA Amplification (LAMP) Assay for Detection of Clostridium difficile Infection (CDI): a Meta-Analysis
نویسندگان
چکیده
Amplification (LAMP) Assay for Detection of Clostridium difficile Infection (CDI): a Meta-Analysis Aaron Lloyd, BS; Priyaleela Thota, MD; Chaitanya Pant, MD; Vinay Pasupuleti, MD, PhD; Adrian V. Hernandez, MD, PhD; Curtis J. Donskey, MD; Abhishek Deshpande, MD, PhD; School of Medicine, Case Western Reserve School of Medicine, Cleveland, OH; Infectious Disease, Case Western Reserve Medical School, Cleveland, OH; Kansas University Medical Center, Kansas City, KS; Medicine, Case Western Reserve University, Cleveland, OH; Quantitative Health Sciences, Cleveland Clinic, Cleveland, OH; Infectious Diseases, Case Western Reserve University, Cleveland, OH; Infectious Diseases, Cleveland Clinic, Cleveland, OH; Medicine, Cleveland Clinic, Cleveland, OH
منابع مشابه
Accuracy of loop-mediated isothermal amplification for the diagnosis of Clostridium difficile infection: a systematic review.
Loop-mediated isothermal DNA amplification (LAMP) is currently used as standalone diagnostic test for C. difficile infection (CDI). We assessed the diagnostic accuracy of LAMP for the diagnosis of CDI. We searched 5 databases to identify studies that compared LAMP with culture cytotoxicity neutralization assay or anaerobic toxigenic culture (TC) of C. difficile. We used the random-effects model...
متن کاملMolecular techniques for diagnosis of Clostridium difficile infection: systematic review and meta-analysis.
OBJECTIVE To assess the usefulness of 2 rapid molecular diagnostic techniques, polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP), in Clostridium difficile infection (CDI). METHODS We conducted a systematic review and meta-analysis to evaluate the accuracy of PCR and LAMP in diagnosis of CDI, including studies that used toxigenic culture or cytotoxicity assay as...
متن کاملDiagnostic accuracy of loop-mediated isothermal amplification in detection of Clostridium difficile in stool samples: a meta-analysis
INTRODUCTION Clostridium difficile infection (CDI) remains a diagnostic challenge for clinicians. More recently, loop-mediated isothermal amplification (LAMP) has become readily available for the diagnosis of CDI, and many studies have investigated the usefulness of LAMP for rapid and accurate diagnosis of CDI. However, the overall diagnostic accuracy of LAMP for CDI remains unclear. In this me...
متن کاملLoop-Mediated Isothermal Amplification (LAMP) for the Rapid Diagnosis of Herpes Simplex Virus Type 1 (HSV-1)
Background and Aims: considering difficulties in usual laboratory methods in detection of viral infections, improved DNA-based diagnostic techniques are more reliable. Loop mediated isothermal amplification method (LAMP) is a nucleic acid amplification method that amplifies DNA using six primers which has been developed to diagnose viruses as a rapid and high efficiency test. In this study, the...
متن کاملA reverse transcriptase-loop mediated isothermal amplification assay (RT-LAMP) for rapid detection of bovine viral diarrhea virus 1 and 2
Bovine viral diarrhea virus (BVDV) is a pathogen that infects cattle, and is globally important. It causes substantial financial losses to the livestock industry. In the current study, a one-step reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay was set up for rapid and efficient detection of BVDV. For this purpose, four primers were designed to recognize six distinct...
متن کامل